Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain

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Publication Type:

Journal Article

Authors:

Mulholland, V.; Schlenzig, A.; MacAskill, G. A.; Green, S.

Source:

Forest Pathology, p.Early view (2013)

URL:

http://onlinelibrary.wiley.com/doi/10.1111/efp.12058/abstract

Abstract:

A TaqMan real-time PCR assay was developed for Phytophthora austrocedrae, an emerging pathogen causing severe damage to juniper in Britain. The primers amplified DNA of the target pathogen down to 1 pg of extracted DNA, in both the presence and absence of host DNA, but did not amplify any of the non-target Phytophthora and fungal species tested. The assay provides a useful tool for screening juniper populations for the disease.

Related Species: 
austrocedrae