TY - JOUR T1 - Development of a quantitative real-time PCR assay for the detection of Phytophthora austrocedrae, an emerging pathogen in Britain JF - Forest Pathology Y1 - 2013 A1 - Mulholland, V. A1 - Schlenzig, A. A1 - MacAskill, G. A. A1 - Green, S. AB -

A TaqMan real-time PCR assay was developed for Phytophthora austrocedrae, an emerging pathogen causing severe damage to juniper in Britain. The primers amplified DNA of the target pathogen down to 1 pg of extracted DNA, in both the presence and absence of host DNA, but did not amplify any of the non-target Phytophthora and fungal species tested. The assay provides a useful tool for screening juniper populations for the disease.

UR - http://onlinelibrary.wiley.com/doi/10.1111/efp.12058/abstract JO - For. Path. ER -