Development of a lab-on-a-chip device for diagnosis of plant pathogens
Sandra Julich, Marko Riedel, Mark Kielpinski, Matthias Urban, Robert Kretschmer, Stefan Wagner, Wolfgang Fritzsche, Thomas Henkel, Robert Möller, Sabine Werres
Biosensors and Bioelectronics, Volume 26, Issue 10, 15 June 2011, Pages 4070-4075
A lab-on-a-chip system for rapid nucleic acid-based analysis was developed that can be applied for diagnosis of selected Phytophthora species as a first example for use in plant pathology. All necessary polymerase chain reaction process (PCR) and hybridization steps can be performed consecutively within a single chip consisting of two components, an inflexible and a flexible one, with integrated microchannels and microchambers. Data from the microarray is collected from a simple electrical measurement that is based on elementary silver deposition by enzymatical catalyzation. Temperatures in the PCR and in the hybridization zone are managed by two independent Peltier elements. The chip will be integrated in a compact portable system with a pump and power supply for use on site. The specificity of the lab-on-a-chip system could be demonstrated for the tested five Phytophthora species. The two Pythium species gave signals below the threshold. The results of the electrical detection of the microarray correspond to the values obtained with the control method (optical grey scale analysis).