<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Kunadiya, Manisha B.</style></author><author><style face="normal" font="default" size="100%">Dunstan, William D.</style></author><author><style face="normal" font="default" size="100%">White, Diane</style></author><author><style face="normal" font="default" size="100%">Hardy, Giles E. St. J.</style></author><author><style face="normal" font="default" size="100%">Grigg, Andrew H.</style></author><author><style face="normal" font="default" size="100%">Burgess, Treena I.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A qPCR Assay for the Detection of Phytophthora cinnamomi Including an mRNA Protocol Designed to Establish Propagule Viability in Environmental Samples</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Disease</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Disease</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-09-2019</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://apsjournals.apsnet.org/doi/10.1094/PDIS-09-18-1641-RE</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">103</style></volume><pages><style face="normal" font="default" size="100%">2443 - 2450</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Phytophthora cinnamomi&lt;/em&gt; causes root and collar rot in many plant species in natural ecosystems and horticulture. A species-specific primer and probe PCIN5 were designed based on a mitochondrial locus encoding subunit 2 of cytochrome c oxidase (&lt;em&gt;cox&lt;/em&gt;2). Eight PCR primers, including three forward and five reverse, were designed and tested in all possible combinations. Annealing temperatures were optimized for each primer pair set to maximize both specificity and sensitivity. Each set was tested against &lt;em&gt;P. cinnamomi&lt;/em&gt; and two closely related clade 7 species, &lt;em&gt;P. parvispora&lt;/em&gt; and &lt;em&gt;P. niederhauseri&lt;/em&gt;. From these tests, five primer pairs were selected based on specificity and, with a species-specific &lt;em&gt;P. cinnamomi&lt;/em&gt; probe, used to develop quantitative real-time PCR (qPCR) assays. The specificity of the two most sensitive qPCR assays was confirmed using the genomic DNA of 29 &lt;em&gt;Phytophthora&lt;/em&gt; isolates, including 17 isolates of 11 species from clade 7, and representative species from nine other clades (all except clade 3). The assay was able to detect as little as 150 ag of &lt;em&gt;P. cinnamomi&lt;/em&gt; DNA and showed no cross-reaction with other &lt;em&gt;Phytophthora&lt;/em&gt; species, except for &lt;em&gt;P. parvispora&lt;/em&gt;, a very closely related species to &lt;em&gt;P. cinnamomi&lt;/em&gt;, which showed late amplification at high DNA concentrations. The efficiency of the qPCR protocol was evaluated with environmental samples including roots and associated soil from plants artificially infected with &lt;em&gt;P. cinnamomi&lt;/em&gt;. Different RNA isolation kits were tested and evaluated for their performance in the isolation of RNA from environmental samples, followed by cDNA synthesis, and qPCR assay. Finally, a protocol was recommended for determining the presence of &lt;em&gt;P. cinnamomi&lt;/em&gt; in recalcitrant environmental samples.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shearer, B. L.</style></author><author><style face="normal" font="default" size="100%">C. E. Crane</style></author><author><style face="normal" font="default" size="100%">A. Cochrane</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Quantification of the susceptibility of the native flora of the South-West Botanical Province, Western Australia, to Phytophthora cinnamomi</style></title><secondary-title><style face="normal" font="default" size="100%">Australian Journal of Botany</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.publish.csiro.au.proxy.library.oregonstate.edu/?paper=BT03131</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">52 (4)</style></volume><pages><style face="normal" font="default" size="100%">435 - 443</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;This study compares, for the first time, variation in estimates of susceptibility of native flora to &lt;em&gt;Phytophthora cinnamomi&lt;/em&gt; Rands among four databases and proposes an estimate of the proportion of the flora of the South-West Botanical Province of Western Australia that is susceptible to the pathogen. Estimates of the susceptibility of south-western native flora to &lt;em&gt;P. cinnamomi&lt;/em&gt; infection were obtained from databases for &lt;em&gt;Banksia&lt;/em&gt; woodland of the Swan Coastal Plain, jarrah (&lt;em&gt;Eucalyptus marginata&lt;/em&gt; Donn. ex Smith) forest, the Stirling Range National Park and Rare and Threatened Flora of Western Australia. For the woodland, forest and national park databases, hosts were naturally infected in uncontrolled diverse natural environments. In contrast, threatened flora were artificially inoculated in a shadehouse environment. Considerable variation occurred within taxonomic units, making occurrence within family and genus poor predictors of species susceptibility. Identification of intra-specific resistance suggests that &lt;em&gt;P. cinnamomi&lt;/em&gt; could be having a strong selection pressure on some threatened flora at infested sites and the populations could shift to more resistant types. Similar estimates of the proportion of species susceptible to &lt;em&gt;P. cinnamomi&lt;/em&gt; among the databases from the wide range of environments suggests that a realistic estimate of species susceptibility to &lt;em&gt;P. cinnamomi&lt;/em&gt; infection in the south-western region has been obtained. The mean of 40% susceptible and 14% highly susceptible equates to 2284 and 800 species of the 5710 described plant species in the South-West Botanical Province susceptible and highly susceptible to &lt;em&gt;P. cinnamomi&lt;/em&gt;, respectively. Such estimates are important for determining the cost of disease to conservation values and for prioritising disease importance and research priorities. &lt;em&gt;P. cinnamomi&lt;/em&gt; in south-western Australia is an unparalleled example of an introduced pathogen with a wide host range causing immense irreversible damage to unique, diverse but mainly susceptible plant communities.&lt;/p&gt;</style></abstract></record></records></xml>