<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Winton, L. M.</style></author><author><style face="normal" font="default" size="100%">Hansen, E.M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular diagnosis of Phytophthora lateralis in trees, water, and foliage baits using multiplex polymerase chain reaction.</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">PHYTOPHTHORA diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">PLANT diseases</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><number><style face="normal" font="default" size="100%">5</style></number><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">275 - 283</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A polymerase chain reaction (PCR)-based protocol for detection of &lt;em&gt;Phytophthora lateralis&lt;/em&gt; in plant tissues and water is described. Base-pair (bp) deletions in both of the ribosomal DNA internal transcribed spacer (ITS) regions in &lt;em&gt;P. lateralis&lt;/em&gt; were used to design complementary PCR primer sequences that amplify a 738 bp fragment only if &lt;em&gt;P. lateralis&lt;/em&gt; DNA is present in the sample. Universal control primers based on conserved sequences of the nuclear ribosomal small subunit are included in a multiplexed reaction, providing an internal check on the procedure. The universal primers amplify an approximately 550 bp fragment that is common to plants, protists, and true fungi. The procedure reliably detects &lt;em&gt;P. lateralis&lt;/em&gt; in cedar stem tissues and in roots. Positive reactions were obtained with as few as 200 &lt;em&gt;P. lateralis&lt;/em&gt; zoospores in water. (English) [ABSTRACT FROM AUTHOR]&lt;/p&gt;</style></abstract></record></records></xml>