<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pérez-Sierra, A.</style></author><author><style face="normal" font="default" size="100%">Kalantarzadeh, M.</style></author><author><style face="normal" font="default" size="100%">Sancisi-Frey, S.</style></author><author><style face="normal" font="default" size="100%">Brasier, C.M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phytophthora siskiyouensis causing stem lesions and cankers on Alnus incana</style></title><secondary-title><style face="normal" font="default" size="100%">New Disease Reports</style></secondary-title><short-title><style face="normal" font="default" size="100%">New Dis. Rep.</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jun-06-2017</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ndrs.org.uk/contents.php?vol=31http://www.ndrs.org.uk/article.php?id=031017</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">17</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;div id=&quot;repbody&quot; class=&quot;clearfix&quot;&gt;&lt;p&gt;In late summer 2013, stem cankers and sparse foliage were reported on European grey alder (&lt;em&gt;Alnus incana&lt;/em&gt;) growing on a 500 ha site recently-planted with broadleaf and coniferous trees in south-west England. A site visit showed that approximately 10% of&amp;nbsp; more than 1000 grey alders (thought to have been imported from Europe and planted in the late 1990s) had symptoms including bleeding stem lesions similar to those caused by &lt;em&gt;Phytophthora alni &lt;/em&gt;(Gibbs &lt;em&gt;et al.&lt;/em&gt;, 2003). In November 2013, samples were collected from stem lesions (Fig. 1), roots (internal lesions tracking-down from stem lesions) and rhizosphere soil from symptom-bearing trees. Tissue from root and stem lesion margins was plated onto &lt;em&gt;Phytophthora &lt;/em&gt;selective medium (SMA) (amended as per Brasier &lt;em&gt;et al.&lt;/em&gt;, 2005) and incubated at 20°C for 48 hrs. Green apples were used as baits for soil samples by inserting a few grams of soil under a flap cut in the side of the apple and incubating for 4-7 days at 20°C. Isolation from developing SMA mycelial cultures and incubated apple baits onto potato dextrose agar (PDA) and carrot agar (CA) was then undertaken.&lt;/p&gt;&lt;p&gt;After 14 days on PDA at 20°C in the dark, colonies exhibited a distinctive stellate growth pattern (Fig. 2a). On CA they had a diffuse 'frosty' appearance (Fig. 2b). On CA, oogonia with predominantly paragynous antheridia and aplerotic oospores were abundant. Partially-caducous sporangia formed when plugs from colonies on CA were submerged in unsterile pond water held at 20°C in the dark. Sporangia (46-51 μm wide) were semi-papillate and were ovoid, reniform, elongated or irregular in shape (Fig. 3). Sporangial morphology and dimensions corresponded to &lt;em&gt;Phytophthora siskiyouensis&lt;/em&gt; (Reeser &lt;em&gt;et al&lt;/em&gt;., 2007) and sequences of ITS and &lt;em&gt;cox&lt;/em&gt;II regions supported this identification (GenBank Accession Nos. KP207601 and KP207602).&amp;nbsp;&lt;/p&gt;&lt;p&gt;One isolate of &lt;em&gt;P. siskiyouensis&lt;/em&gt; was obtained from each of three different symptomatic trees; one from a root lesion, one from a stem lesion and one from associated soil. Koch’s postulates were tested by inoculating two-year-old potted &lt;em&gt;A. incana&lt;/em&gt; saplings with the three isolates. A small wound was made on the stem 10 cm above soil level. A CA plug colonised by &lt;em&gt;P. siskiyouensis&lt;/em&gt; was inserted and the wound was sealed with Parafilm. Nine saplings were inoculated per isolate and maintained at 20°C with a 12-hr-photoperiod. Three control saplings were inoculated with sterile CA plugs. After 20 days, cankers and bleeding were visible externally on all of the trees inoculated with &lt;em&gt;P. siskiyouensis&lt;/em&gt; (Fig. 4). The bark was peeled away to reveal phloem lesions extending approximately 2-4 cm above and below the inoculation point. No lesions developed on control trees. After re-isolation onto SMA, a &lt;em&gt;Phytophthora &lt;/em&gt;sp. was recovered from all of the inoculated trees but not from the controls and it was identified as &lt;em&gt;P. siskiyouensis &lt;/em&gt;by morphology (Reeser &lt;em&gt;et al&lt;/em&gt;., 2007) and sequencing of the ITS region.&lt;/p&gt;&lt;p&gt;&lt;em&gt;Phytophthora siskiyouensis &lt;/em&gt;is a recently-described species in the USA, isolated from stem lesions on myrtlewood (&lt;em&gt;Umbellularia californica&lt;/em&gt;) and tanoak (&lt;em&gt;Lithocarpus densiflorus&lt;/em&gt;; synonym of&lt;em&gt; Nothocarpus densiflorus&lt;/em&gt;) and from soil and stream water, in south-west Oregon (Reeser &lt;em&gt;et al&lt;/em&gt;., 2007). It has also been reported causing stem lesions on Italian alder (&lt;em&gt;Alnus&lt;/em&gt; &lt;em&gt;cordata&lt;/em&gt;) in California (Rooney-Latham &lt;em&gt;et al&lt;/em&gt;., 2007). This is the first report of &lt;em&gt;P. siskiyouensis&lt;/em&gt; in the UK and, to our knowledge, in Europe, and the first report of &lt;em&gt;P. siskiyouensis&lt;/em&gt; causing stem cankers on &lt;em&gt;A. incana&lt;/em&gt;. Its occurrence within a recently-planted site suggests a possible origin on introduced nursery stock. &lt;em&gt;P. siskiyouensis&lt;/em&gt; has the potential to cause further damage to &lt;em&gt;Alnus&lt;/em&gt; spp. and other plant species in Europe. Its comparative pathogenicity on various alder species is under investigation.&lt;/p&gt;&lt;/div&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pérez-Sierra, A.</style></author><author><style face="normal" font="default" size="100%">López-García, C.</style></author><author><style face="normal" font="default" size="100%">León, M.</style></author><author><style face="normal" font="default" size="100%">García-Jiménez, J.</style></author><author><style face="normal" font="default" size="100%">Abad-Campos, P.</style></author><author><style face="normal" font="default" size="100%">T. Jung</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Previously unrecorded low-temperature Phytophthora species associated with Quercus decline in a Mediterranean forest in eastern Spain</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">08/2013</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/efp.12037</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">43</style></volume><pages><style face="normal" font="default" size="100%">331–339</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Oak decline has been a serious problem in Europe since the beginning of the twentieth century. In south-west Spain, &lt;em&gt; Quercus ilex&lt;/em&gt; and &lt;em&gt; Q.&amp;nbsp;suber&lt;/em&gt; are the main affected species, and their decline has been associated with &lt;em&gt; Phytophthora cinnamomi&lt;/em&gt;. During the last 10&amp;nbsp;years, a severe decline of &lt;em&gt; Q.&amp;nbsp;ilex&lt;/em&gt; and &lt;em&gt; Q.&amp;nbsp;faginea&lt;/em&gt; accompanied by a significant decrease in the production of acorns affecting natural regeneration was observed in the eastern part of the Iberian Peninsula. Therefore, the aim of this study was to investigate the possible involvement of &lt;em&gt;Phytophthora&lt;/em&gt; spp. in the decline. A forest in the Natural Park ‘Carrascar de la Font Roja’ in Comunidad Valenciana (eastern Spain), which is dominated by &lt;em&gt; Q.&amp;nbsp;ilex&lt;/em&gt; and &lt;em&gt; Q.&amp;nbsp;faginea&lt;/em&gt;, was surveyed during 2010–2011. Symptomatic trees showed thinning and dieback of the crown, withering of leaves and death. An extensive loss of both lateral small woody roots and fine roots and callusing or open cankers on suberized roots were observed. Soil samples containing fine roots were baited using both &lt;em&gt; Q.&amp;nbsp;robur&lt;/em&gt; leaves and apple fruits. Six &lt;em&gt; Phytophthora&lt;/em&gt; species were isolated: &lt;em&gt; P.&amp;nbsp;cryptogea, P.&amp;nbsp;gonapodyides, P.&amp;nbsp;megasperma, P.&amp;nbsp;quercina, P.&amp;nbsp;psychrophila&lt;/em&gt; and &lt;em&gt; P.&amp;nbsp;syringae&lt;/em&gt;. These are the first records of &lt;em&gt; P.&amp;nbsp;quercina&lt;/em&gt; and &lt;em&gt; P.&amp;nbsp;psychrophila&lt;/em&gt; on &lt;em&gt;Q&lt;/em&gt;. &lt;em&gt; faginea&lt;/em&gt;, of &lt;em&gt; P.&amp;nbsp;quercina&lt;/em&gt; in Spain and of &lt;em&gt; P.&amp;nbsp;psychrophila&lt;/em&gt; in mainland Spain. A soil infestation trial was conducted for 6&amp;nbsp;months under controlled conditions with 1-year-old seedlings of &lt;em&gt; Q.&amp;nbsp;ilex&lt;/em&gt; and &lt;em&gt; Q.&amp;nbsp;faginea&lt;/em&gt;. &lt;em&gt; Phytophthora cinnamomi&lt;/em&gt; was included in the pathogenicity test for comparison. The results showed that &lt;em&gt; Q.&amp;nbsp;ilex&lt;/em&gt; seedlings were generally more susceptible to infection than &lt;em&gt; Q.&amp;nbsp;faginea&lt;/em&gt; with &lt;em&gt; P.&amp;nbsp;cinnamomi&lt;/em&gt; being the most aggressive pathogen to both oak species. The two most commonly isolated &lt;em&gt; Phytophthora&lt;/em&gt; species, &lt;em&gt; P.&amp;nbsp;quercina&lt;/em&gt; and &lt;em&gt; P.&amp;nbsp;psychrophila&lt;/em&gt;, also proved their pathogenicity towards both &lt;em&gt; Q.&amp;nbsp;ilex&lt;/em&gt; and &lt;em&gt; Q.&amp;nbsp;faginea&lt;/em&gt;.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pérez-Sierra, A.</style></author><author><style face="normal" font="default" size="100%">Álvarez, L. A.</style></author><author><style face="normal" font="default" size="100%">Vercauteren, A.</style></author><author><style face="normal" font="default" size="100%">Heungens, K.</style></author><author><style face="normal" font="default" size="100%">Abad-Campos, P.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic diversity, sensitivity to phenylamide fungicides and aggressiveness of Phytophthora ramorum on Camellia, Rhododendron and Viburnum plants in Spain</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">fungicide resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">host origin</style></keyword><keyword><style  face="normal" font="default" size="100%">mefenoxam</style></keyword><keyword><style  face="normal" font="default" size="100%">metalaxyl</style></keyword><keyword><style  face="normal" font="default" size="100%">multilocus genotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Sudden oak death</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.1365-3059.2011.02485.x</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">6</style></number><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">60</style></volume><pages><style face="normal" font="default" size="100%">1069–1076</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Phytophthora ramorum&lt;/em&gt; has been detected in official plant health surveys on Rhododendron, Viburnum and Camellia in ornamental nurseries in northern Spain since 2003. A collection of 94 isolates of &lt;em&gt;P. ramorum&lt;/em&gt; was obtained from 2003 to 2008 from plants with symptoms at different geographical locations. Isolates were identified based on morphology and sequence of the rDNA ITS region. Mating type, genetic variation, sensitivity to phenylamide fungicides and aggressiveness of these isolates were determined. All isolates belonged to the A1 mating type, ruling out the possibility of genetic recombination. Seven microsatellite markers were used to study genetic diversity; three out of the seven microsatellite markers were polymorphic within the Spanish population of &lt;em&gt;P. ramorum&lt;/em&gt;. This study confirms that all Spanish isolates of &lt;em&gt;P. ramorum&lt;/em&gt; belonged to the EU1 lineage. Twelve intralineage genotypes were detected, five that are unique to Spain (EU1MG38, EU1MG41, EU1MG37, EU1MG39 and EU1MG40) and seven that are also present in at least one other European country (EU1MG1, EU1MG29, EU1MG22, EU1MG13, EU1MG2, EU1MG18 and EU1MG26). Genotypes EU1MG37, EU1MG39 and EU1MG40 were isolated from Rhododendron from one region; EU1MG38 and EU1MG41 were isolated from Camellia from two different regions. Isolates of genotype EU1MG38 were resistant to metalaxyl and mefenoxam. The level of genetic diversity within the Spanish population of &lt;em&gt;P. ramorum&lt;/em&gt; is limited and indicates a relatively recent clonal expansion.&lt;/p&gt;</style></abstract></record></records></xml>