<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Katherine J. Hayden</style></author><author><style face="normal" font="default" size="100%">David Rizzo</style></author><author><style face="normal" font="default" size="100%">Justin Tse</style></author><author><style face="normal" font="default" size="100%">Garbelotto, Matteo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection and quantification of Phytophthora ramorum from California forests using a real-time polymerase chain reaction assay-0</style></title><secondary-title><style face="normal" font="default" size="100%">Phytopathology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://apsjournals.apsnet.org/doi/abs/10.1094/PHYTO.2004.94.10.1075</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">94</style></volume><pages><style face="normal" font="default" size="100%">1075-1083</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The timely and accurate detection of pathogens is a critical aid in the study of the epidemiology and biology of plant diseases. In the case of regulated organisms, the availability of a sensitive and reliable assay is essential when trying to achieve early detection of the pathogen. We developed and tested a real-time, nested polymerase chain reaction (PCR) assay for the detection of &lt;em&gt;Phytophthora ramorum&lt;/em&gt;, causal agent of sudden oak death. This technique then was implemented as part of a widespread environmental screen throughout California. The method here described is sensitive, detecting less than 12 fg of pathogen DNA, and is specific for &lt;em&gt;P. ramorum&lt;/em&gt; when tested across 21 &lt;em&gt;Phytophthora spp&lt;/em&gt;. Hundreds of symptomatic samples from 33 sites in 14 California counties were assayed, resulting in the discovery of 10 new host species and 23 infested areas, including 4 new counties. With the exception of a single host, PCR-based discovery of new hosts and infested areas always was confirmed by traditional pathogen isolations and inoculation studies. Nonetheless, molecular diagnostics were key in early pathogen detection, and steered the direction of further research on this newly discovered and generalist &lt;em&gt;Phytophthora&lt;/em&gt; species.&lt;/p&gt;</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Katherine J. Hayden</style></author><author><style face="normal" font="default" size="100%">Alejandro Nettel</style></author><author><style face="normal" font="default" size="100%">Richard S. Dodd</style></author><author><style face="normal" font="default" size="100%">Garbelotto, Matteo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Will all the trees fall? Variable resistance to an introduced forest disease in a highly susceptible host</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Ecology and Management</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Sudden oak death</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/B6T6X-52F7TF6-1/2/55216e9ccfc0fafe0035e3d3f20ff81b</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">11</style></number><volume><style face="normal" font="default" size="100%">261</style></volume><pages><style face="normal" font="default" size="100%">1781 - 1791</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Although tanoak (&lt;em&gt;Notholithocarpus densiflorus syn. Lithocarpus densiflorus&lt;/em&gt;) is the species most affected by the introduced pathogen &lt;em&gt;Phytophthora ramorum&lt;/em&gt;, with demonstrable risk of extirpation, little is known about the origin, range or structuring of the tree’s susceptibility. We examined variation in resistance to &lt;em&gt;P. ramorum&lt;/em&gt; using a wound inoculation assay of detached leaves from trees at five geographically separated sites, and a non-wound inoculation assay on twigs from trees at two sites. The structure of variation in resistance was compared to the structure at nine nuclear microsatellite markers. Resistance varied quantitatively, with 23% and 12% of the variation among individuals and populations, respectively. There was a significant correlation between resistance in detached leaves and lesion size in non-wounding twig inoculations. Among-population genetic diversity at nine microsatellite loci was weakly structured but significantly non-zero, with 9.5% of variation among populations. Within-population neutral genetic diversity was a poor predictor of resistance, and estimates of phenotypic distances for resistance were no greater than neutral genetic distances. The limited phenotypic and genetic structure we found indicates that tanoaks at all study sites are susceptible, and there is no evidence of prior selection for disease resistance. We conclude that tanoak populations across the species’ range are at risk, but local disease dynamics will depend on both host genetics and environmental conditions.&lt;/p&gt;</style></abstract></record></records></xml>