<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Schubert, R.</style></author><author><style face="normal" font="default" size="100%">Bahnweg, G.</style></author><author><style face="normal" font="default" size="100%">Nechwatal, J.</style></author><author><style face="normal" font="default" size="100%">T. Jung</style></author><author><style face="normal" font="default" size="100%">Cooke, D. E. L.</style></author><author><style face="normal" font="default" size="100%">Duncan, J. M.</style></author><author><style face="normal" font="default" size="100%">Muller-Starck, G.</style></author><author><style face="normal" font="default" size="100%">Langebartels, C.</style></author><author><style face="normal" font="default" size="100%">Sandermann, H. Jr2</style></author><author><style face="normal" font="default" size="100%">W. Osswald</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Forest Pathol</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">1999</style></year><pub-dates><date><style  face="normal" font="default" size="100%">06/1999</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">29</style></volume><pages><style face="normal" font="default" size="100%">169 - 188</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected &lt;em&gt;Phytophthora&lt;/em&gt; species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with &lt;em&gt;Phytophthora citricola.&lt;/em&gt; The &lt;em&gt;Phytophthora cambivora&lt;/em&gt; specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the &lt;em&gt;Phytophthora quercina&lt;/em&gt; specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other &lt;em&gt;Phytophthora&lt;/em&gt; species, &lt;em&gt;Pythium, Xerocomus, Hebeloma, Russula&lt;/em&gt;, and &lt;em&gt;Armillaria.&lt;/em&gt; Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (&lt;em&gt;P. cambivora&lt;/em&gt;), 4pg (&lt;em&gt;P. quercina&lt;/em&gt;), and 2pg (&lt;em&gt;P. citricola&lt;/em&gt;) target DNA. This diagnostic PCR system was able to detect &lt;em&gt;P. citricola, P. quercina&lt;/em&gt;, and &lt;em&gt;P. cambivora&lt;/em&gt; in seedlings of pendunculate oak (&lt;em&gt;Quercus robur&lt;/em&gt;) and European beech (&lt;em&gt;Fagus sylvatica&lt;/em&gt;) which were artificially infected under controlled conditions.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue></record></records></xml>