<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hardoim, P. R.</style></author><author><style face="normal" font="default" size="100%">Guerra, R.</style></author><author><style face="normal" font="default" size="100%">Rosa da Costa, A. M.</style></author><author><style face="normal" font="default" size="100%">Serrano, M. S.</style></author><author><style face="normal" font="default" size="100%">Sanchez, M. E.</style></author><author><style face="normal" font="default" size="100%">Coelho, A. C.</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Stenlid, J.</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Temporal metabolic profiling of the Quercus suber - Phytophthora cinnamomi system by middle-infrared spectroscopy</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">For. Path.</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-04-2016</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1111/efp.2016.46.issue-2http://doi.wiley.com/10.1111/efp.12229http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fefp.12229</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">46</style></volume><pages><style face="normal" font="default" size="100%">122 - 133</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The oomycete &lt;em&gt;Phytophthora cinnamomi&lt;/em&gt; is an aggressive plant pathogen, detrimental to many ecosystems including cork oak (&lt;em&gt;Quercus suber&lt;/em&gt;) stands, and can inflict great losses in one of the greatest ‘hotspots’ for biodiversity in the world. Here, we applied Fourier transform-infrared (FT-IR) spectroscopy combined with chemometrics to disclose the metabolic patterns of cork oak roots and &lt;em&gt;P.&amp;nbsp;cinnamomi&lt;/em&gt; mycelium during the early hours of the interaction. As early as 2&amp;nbsp;h post-inoculation (hpi), cork oak roots showed altered metabolic patterns with significant variations for regions associated with carbohydrate, glycoconjugate and lipid groups when compared to mock-inoculated plants. These variations were further extended at 8&amp;nbsp;hpi. Surprisingly, at 16&amp;nbsp;hpi, the metabolic changes in inoculated and mock-inoculated plants were similar, and at 24&amp;nbsp;hpi, the metabolic patterns of the regions mentioned above were inverted when compared to samples collected at 8&amp;nbsp;hpi. Principal component analysis of the FT-IR spectra confirmed that the metabolic patterns of inoculated cork oak roots could be readily distinguished from those of mock-inoculated plants at 2, 8 and 24&amp;nbsp;hpi, but not at 16&amp;nbsp;hpi. FT-IR spectral analysis from mycelium of &lt;em&gt;P.&amp;nbsp;cinnamomi&lt;/em&gt; exposed to cork oak root exudates revealed contrasting variations for regions associated with protein groups at 16 and 24&amp;nbsp;h post-exposure (hpe), whereas carbohydrate and glycoconjugate groups varied mainly at 24&amp;nbsp;hpe. Our results revealed early alterations in the metabolic patterns of the host plant when interacting with the biotrophic pathogen. In addition, the FT-IR technique can be successfully applied to discriminate infected cork oak plants from mock-inoculated plants, although these differences were dynamic with time. To a lesser extent, the metabolic patterns of &lt;em&gt;P.&amp;nbsp;cinnamomi&lt;/em&gt; were also altered when exposed to cork oak root exudates.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record></records></xml>