<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Blomquist, C. L.</style></author><author><style face="normal" font="default" size="100%">Yakabe, L. E.</style></author><author><style face="normal" font="default" size="100%">Soriano, M. C.</style></author><author><style face="normal" font="default" size="100%">Negrete, M. A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">First Report of Leaf Spot Caused by Phytophthora taxon Pgchlamydo on Evergreen Nursery Stock in California</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Disease</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Disease</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-11-2012</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://apsjournals.apsnet.org/doi/10.1094/PDIS-02-12-0221-PDN</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">96</style></volume><pages><style face="normal" font="default" size="100%">1691 - 1691</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;div class=&quot;hlFld-Abstract&quot;&gt;&lt;div class=&quot;abstractSection&quot;&gt;&lt;p class=&quot;first&quot;&gt;As part of the &lt;em&gt;Phytophthora ramorum&lt;/em&gt; testing program from 2005 through 2007, a &lt;em&gt;Phytophthora&lt;/em&gt; sp. was isolated on PARP-CMA medium (4) at the CDFA lab in Sacramento, CA, from the margin of necrotic spots and tissue suffering from dieback on &lt;em&gt;Arctostaphylos&lt;/em&gt; sp. (manzanita), &lt;em&gt;Camellia&lt;/em&gt; spp., &lt;em&gt;Laurus nobilis&lt;/em&gt; (bay), &lt;em&gt;Buxus sempervirens&lt;/em&gt; (boxwood), &lt;em&gt;Rhododendron&lt;/em&gt; sp., &lt;em&gt;Arbutus unedo&lt;/em&gt; (strawberry tree), and &lt;em&gt;Sequoia sempervirens&lt;/em&gt; (coast redwood). Isolates were collected from Shasta, Contra Costa, San Diego, Solano, Santa Cruz, Alameda, Sacramento, San Joaquin, Monterey, and Los Angeles Counties. Isolates from &lt;em&gt;A. unedo&lt;/em&gt; tissue on PARP medium produced apapillate, obovate sporangia 25 to 80 × 15 to 40 μm (48.0 × 26.9 μm average) and a few isolates produced intercalary and terminal chlamydospores at 22°C (30 to 46 μm diameter, 38.9 μm average). The internal transcribed spacer region (ITS) of rDNA was amplified from four isolates using ITS1 and ITS4 primers as described by White et al. (3) and the amplicons sequenced (GenBank Accession Nos. JQ307188 through JQ307191). BLAST analysis of the amplicons showed 99 to 100% identity with the ITS sequence of &lt;em&gt;Phytophthora&lt;/em&gt; taxon Pgchlamydo from forest streams in Oregon (GenBank Accession No. HM004224) (1). Pathogenicity tests were performed on &lt;em&gt;B. sempervirens, C. sasanqua, L. nobilis&lt;/em&gt;, and &lt;em&gt;A. unedo&lt;/em&gt;. Five plants of each species were inoculated with 6-mm plugs taken from the margin of a 7- to 10-day-old culture grown on V8 juice agar. Plant leaves were wounded with a sterile pushpin and two agar plugs were covered with a freezer tube cap filled with sterile dH&lt;sub&gt;2&lt;/sub&gt;O and clipped to the underside of the leaves with a sterile pin-curl clip (4). Inoculated plants were sprayed with water, covered with plastic bags, and incubated for 2 days, when bags and plugs were removed. Five leaves of each isolate plus five control plugs using V8 juice agar alone were inoculated on each plant. Plants were incubated for 12 days at 18°C (16-h photoperiod). Lesions formed on all inoculated plants, ranging in size from approx. 1 mm on &lt;em&gt;B. sempervirens&lt;/em&gt; to 9.2 × 10.9 mm average on &lt;em&gt;A. unedo&lt;/em&gt;. The lesions on &lt;em&gt;A. unedo&lt;/em&gt; grew into and caused the mid-vein to blacken. The lesion sizes on camellia and bay were larger than those formed on &lt;em&gt;B. sempervirens&lt;/em&gt; and smaller than those formed on &lt;em&gt;A. unedo&lt;/em&gt;, with most lesions surrounded by a dark ring. &lt;em&gt;Phytophthora&lt;/em&gt; taxon Pgchlamydo is associated with leaf lesions on rhododendron and dieback of yew in Minnesota (2). To our knowledge, this is the first report of &lt;em&gt;Phytophthora&lt;/em&gt; taxon Pgchlamydo causing disease in camellia, bay, strawberry tree, and boxwood in California. &lt;em&gt;Phytophthora&lt;/em&gt; taxon Pgchlamydo causes damage that is indistinguishable from the quarantine pest, &lt;em&gt;P. ramorum&lt;/em&gt; (4).&lt;/p&gt;&lt;p class=&quot;last&quot;&gt;&lt;em&gt;References&lt;/em&gt;: (1) P. W. Reeser et al. Mycologia 103:22, 2011. (2) B. W. Schwingle and R. A. Blanchette. Plant Dis. 92:642, 2008. (3) T. J. White et al. PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds., Academic Press, San Diego, 1990. (4) L. E. Yakabe et al. Plant Dis. 93:883, 2009.&lt;/p&gt;&lt;/div&gt;&lt;/div&gt;</style></abstract><issue><style face="normal" font="default" size="100%">11</style></issue></record></records></xml>