<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Shelley, B. A.</style></author><author><style face="normal" font="default" size="100%">Luster, D. G.</style></author><author><style face="normal" font="default" size="100%">Garrett, W. M.</style></author><author><style face="normal" font="default" size="100%">McMahon, M. B.</style></author><author><style face="normal" font="default" size="100%">Widmer, T. L.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effects of temperature on germination of sporangia, infection and protein secretion by Phytophthora kernoviae</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Pathol</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2018</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-04-2018</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://doi.org/10.1111/ppa.12782</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">67</style></volume><pages><style face="normal" font="default" size="100%">719 - 728</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Phytophthora kernoviae&lt;/em&gt; is a pathogen on a wide range of plants, but little is known of optimal infection conditions. &lt;em&gt;Rhododendron ponticum&lt;/em&gt; leaves were inoculated with six different isolates of &lt;em&gt;P.&amp;nbsp;kernoviae&lt;/em&gt; sporangia and incubated at different temperatures from 10 to 28&amp;nbsp;°C. After 1&amp;nbsp;week, lesion development and pathogen recovery were only observed from all isolates at 15 and 20&amp;nbsp;°C and a few isolates at 10&amp;nbsp;°C. In an experiment with temperatures ranging from 20 to 25&amp;nbsp;°C, lesion development and pathogen recovery on &lt;em&gt;R.&amp;nbsp;ponticum&lt;/em&gt;,&lt;em&gt; Magnolia stellata&lt;/em&gt; and &lt;em&gt;Viburnum tinus&lt;/em&gt; occurred consistently at 20 and 21&amp;nbsp;°C, was limited at 22&amp;nbsp;°C, and did not occur at 23&amp;nbsp;°C and above. There was no difference in sporangia and zoospore germination at 20–25&amp;nbsp;°C. In a temperature fluctuation experiment, the necrotic area of inoculated &lt;em&gt;R.&amp;nbsp;ponticum&lt;/em&gt; leaves increased with longer incubation at 20&amp;nbsp;°C and decreased with longer incubation at 24&amp;nbsp;°C. Crude extracts of secreted proteins from &lt;em&gt;P.&amp;nbsp;kernoviae&lt;/em&gt; cultures grown at 20 and 24&amp;nbsp;°C were compared to determine any effects of temperature on pathogenicity. When spot tested on &lt;em&gt;R.&amp;nbsp;ponticum&lt;/em&gt; leaves, crude protein suspensions from cultures grown at 20&amp;nbsp;°C induced necrosis, while proteins from cultures grown at 24&amp;nbsp;°C did not. Proteomic analysis confirmed that a 10&amp;nbsp;kDa protein secreted at both 20 and 24&amp;nbsp;°C shared sequence homology to the conserved domains of known elicitins of other &lt;em&gt;Phytophthora&lt;/em&gt; spp. The protein secreted at 20&amp;nbsp;°C that was responsible for necrosis has not been identified.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue></record></records></xml>