<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Tooley, Paul W.</style></author><author><style face="normal" font="default" size="100%">Browning, Marsha</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The Effect of Exposure to Decreasing Relative Humidity on the Viability of Phytophthora ramorum sporangia</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Phytopathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">J Phytopathol</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Aug 2016</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://doi.org/10.1111/jph.12506 </style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">164</style></volume><pages><style face="normal" font="default" size="100%">874 - 881</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Sporangia of three isolates of &lt;em&gt;Phytophthora ramorum&lt;/em&gt; representing three different clonal lineages were subjected to relative humidity (RH) levels between 80 and 100% for exposure periods ranging from 1 to 24&amp;nbsp;h at 20°C in darkness. Plastic containers (21.5&amp;nbsp;×&amp;nbsp;14.5&amp;nbsp;×&amp;nbsp;5&amp;nbsp;cm) were used as humidity chambers with 130&amp;nbsp;ml of glycerine solution added to each container. Glycerine concentrations corresponded to 100, 95, 90, 85 and 80% RH based on refractive index measurements. Sporangia suspensions were pipeted onto nitrile mesh squares (1.5&amp;nbsp;×&amp;nbsp;1.5&amp;nbsp;cm, 15 micron pore size) which were placed in the humidity chambers and incubated at 20°C in darkness. Following exposure periods of 1, 2, 4, 8, 12 and 24&amp;nbsp;h, mesh squares were inverted onto Petri dishes of selective medium and sporangia germination assessed after 24 and 48&amp;nbsp;h. At 100% RH, we observed a mean value of 88% germination after 1&amp;nbsp;h exposure declining to 18% germination following 24&amp;nbsp;h incubation. At 95% RH, a steeper decline in germination was noted, with means ranging from 79% at 1&amp;nbsp;h to less than 1% at 24&amp;nbsp;h exposure. At 90% RH, no germination was noted after 8 or more h exposure, and values were 57%, 22% and 3% germination for the 1, 2 and 4&amp;nbsp;h exposures, respectively. Germination was only observed at 1&amp;nbsp;h exposure for both the 85% RH treatment (52% germination) and the 80% RH treatment (38% germination). The three isolates responded similarly&amp;nbsp;over the range of RH values tested. The germination response of &lt;em&gt;P.&amp;nbsp;ramorum&lt;/em&gt; sporangia to RH values between 80% and 100% was comparable to that reported for other &lt;em&gt;Phytophthora&lt;/em&gt; species. Knowledge of conditions that affect &lt;em&gt;P&lt;/em&gt;.&amp;nbsp;&lt;em&gt;ramorum&lt;/em&gt; sporangia germination can shed light on pathogenesis and epidemic potential and lead to improved control recommendations.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">11-12</style></issue></record></records></xml>