<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Santos, A. F. dos</style></author><author><style face="normal" font="default" size="100%">Luz, E. D. M. N.</style></author><author><style face="normal" font="default" size="100%">Souza, J. T.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">First report of Phytophthora boehmeriae on black wattle in Brazil</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Pathology</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-12-2006</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01462.x/full</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">55</style></volume><pages><style face="normal" font="default" size="100%">813 - 813</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Black wattle (&lt;em&gt;Acacia mearnsii&lt;/em&gt;) is an Australian tree species cultivated in several countries. In Brazil, particularly in Rio Grande do Sul State (RS), approximately 140&amp;nbsp;000 ha are cultivated for tannin extraction (&lt;a class=&quot;link__reference js-link__reference&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01462.x/full#b1&quot; rel=&quot;references:#b1&quot;&gt;Dos Santos &lt;em&gt;et&amp;nbsp;al&lt;/em&gt;., 2005&lt;/a&gt;).&lt;/p&gt;&lt;p&gt;The gummosis complex, which has &lt;em&gt;Phytophthora nicotianae&lt;/em&gt; as one of the causal agents, is the main disease of black wattle in Brazil and is characterised by lesions at the trunk base with gum exudation (&lt;a class=&quot;link__reference js-link__reference&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01462.x/full#b1&quot; rel=&quot;references:#b1&quot;&gt;Dos Santos &lt;em&gt;et&amp;nbsp;al&lt;/em&gt;., 2005&lt;/a&gt;). Different symptoms were observed in plantations at the municipality of Piratini, RS (southern Brazil) and were characterised by dark lesions without gum exudation on trunks of 3- to 4-year-old wattle trees up to 10 m height. Isolates of &lt;em&gt;Phytophthora&lt;/em&gt; spp. were obtained from the lesions and deposited in the Brazilian collection of &lt;em&gt;Phytophthora&lt;/em&gt; species, under accession numbers CBP 307, 308 and 309. Sporangia of all isolates were ovoid to spherical, papillate and caducous, measuring 35 µm ± 1·42 × 30 µm ± 1·37, with a length/width ratio of 1·16:1, mean depth of papillae of 4·83 µm ± 0·04, and pore exit of 4·69 µm ± 0·04. The isolates were homothallic, forming plerotic oospores with smooth walls and amphigynous antheridia. The ITS sequences obtained for isolates CBP 307 (&lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: AY428533&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=AY428533&quot; target=&quot;_blank&quot;&gt;AY428533&lt;/a&gt;), CBP 308 (&lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: AY428534&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=AY428534&quot; target=&quot;_blank&quot;&gt;AY428534&lt;/a&gt;), and CBP 309 (&lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: AY428535&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=AY428535&quot; target=&quot;_blank&quot;&gt;AY428535&lt;/a&gt;) were identical and most closely matched those of two isolates of &lt;em&gt;P. boehmeriae &lt;/em&gt;&lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: KACC40173&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=KACC40173&quot; target=&quot;_blank&quot;&gt;KACC40173&lt;/a&gt; (&lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: AY228076&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=AY228076&quot; target=&quot;_blank&quot;&gt;AY228076&lt;/a&gt;) from Korea and &lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: SCRP23&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=SCRP23&quot; target=&quot;_blank&quot;&gt;SCRP23&lt;/a&gt; (&lt;a class=&quot;accessionId&quot; title=&quot;Link to external resource: DQ297406&quot; href=&quot;http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&amp;amp;db=Nucleotide&amp;amp;dopt=GenBank&amp;amp;term=DQ297406&quot; target=&quot;_blank&quot;&gt;DQ297406&lt;/a&gt;) from China. This and the morphological similarity (&lt;a class=&quot;link__reference js-link__reference&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01462.x/full#b2&quot; rel=&quot;references:#b2&quot;&gt;Erwin &amp;amp; Ribeiro, 1996&lt;/a&gt;) suggest that these isolates are &lt;em&gt;P. boehmeriae&lt;/em&gt;. However, seven clear single base pair differences were noted between the Brazilian and other &lt;em&gt;P. boehmeriae&lt;/em&gt; isolates. This, combined with isozyme variation (&lt;a class=&quot;link__reference js-link__reference&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01462.x/full#b3&quot; rel=&quot;references:#b3&quot;&gt;Oudemans &amp;amp; Coffey, 1991&lt;/a&gt;) suggests that further studies are needed to confirm the taxonomic status of &lt;em&gt;P. boehmeriae&lt;/em&gt;.&lt;/p&gt;&lt;p&gt;Pathogenicity tests were done by inoculating five 1-year-old wattle plants with 7 mm mycelial discs of 5-day old cultures of the three isolates used in this study. The mycelial discs were placed in 7 mm diameter holes made in the bark with a cork borer, at 5 cm above the soil. Plants were maintained at approximately 25°C and were assessed 45 days after inoculation. All three isolates were pathogenic to black wattle and were re-isolated from the lesions.&lt;/p&gt;&lt;p&gt;&lt;em&gt;Phytophthora boehmeriae&lt;/em&gt; was reported as one of the causal agents of the gummosis complex on black wattle in South Africa (&lt;a class=&quot;link__reference js-link__reference&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2006.01462.x/full#b4&quot; rel=&quot;references:#b4&quot;&gt;TPCP, 2004&lt;/a&gt;) and is of quarantine importance for the citrus industry in Brazil. This is the first report of the involvement of &lt;em&gt;P. boehmeriae&lt;/em&gt; in the aetiology of the gummosis complex of black wattle in Brazil.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue></record></records></xml>