<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Nowakowska, J. A.</style></author><author><style face="normal" font="default" size="100%">Malewski, T.</style></author><author><style face="normal" font="default" size="100%">Tereba, A.</style></author><author><style face="normal" font="default" size="100%">Oszako, T.</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Belbahri, L.</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Rapid diagnosis of pathogenic Phytophthora species in soil by real-time PCR</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">For. Path.</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-07-2016</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/efp.12303</style></url></web-urls></urls><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Real-time PCR assays based on the TaqMan system and using ITS sequences were developed for the identification of &lt;em&gt;Phytophthora&lt;/em&gt; species, including &lt;em&gt;P.&amp;nbsp;cactorum, P.&amp;nbsp;megasperma, P.&amp;nbsp;plurivora, P.&amp;nbsp;pseudosyringae&lt;/em&gt; and &lt;em&gt;P.&amp;nbsp;quercina&lt;/em&gt;, all of which are currently causing significant damage to roots of forest trees in both managed stands and natural ecosystems. Total genomic DNA was extracted from mycelia of aforementioned &lt;em&gt;Phytophthora&lt;/em&gt; isolates. Species-specific primers for &lt;em&gt;P.&amp;nbsp;cactorum, P.&amp;nbsp;megasperma&lt;/em&gt;,&lt;em&gt; P.&amp;nbsp;plurivora&lt;/em&gt;,&lt;em&gt; P.&amp;nbsp;pseudosyringae&lt;/em&gt; and &lt;em&gt;P.&amp;nbsp;quercina&lt;/em&gt; were designed based on ITS sequences of rDNA. The amplification efficiency of target DNA varied from 93.1% (&lt;em&gt;P.&amp;nbsp;pseudosyringae&lt;/em&gt;) to 106.8% (&lt;em&gt;P.&amp;nbsp;quercina&lt;/em&gt;). The limit of the detection was calculated as 100 – 1,000&amp;nbsp;fg DNA, depending on the &lt;em&gt;Phytophthora&lt;/em&gt; species. In mixed soil samples, all &lt;em&gt;Phytophthora&lt;/em&gt; species were detected for Ct values shifted by 0.7 – 2.1 cycles. Based on these real-time PCR assays we were able to identify the five &lt;em&gt;Phytophthora&lt;/em&gt; species. These techniques will be of value in the identification of these pathogens, which may cause up to 80 – 90% fine root loss in oak stands.&lt;/p&gt;</style></abstract></record></records></xml>