<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Català, S.</style></author><author><style face="normal" font="default" size="100%">Berbegal, M.</style></author><author><style face="normal" font="default" size="100%">Pérez-Sierra, A.</style></author><author><style face="normal" font="default" size="100%">Abad-Campos, P.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metabarcoding and development of new Real-time specific assays reveal Phytophthora species diversity in Holm Oak forests in eastern Spain</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Pathol</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-04-2016</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1111/ppa.12541</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">66</style></volume><pages><style face="normal" font="default" size="100%">115–123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The evergreen holm oaks (&lt;em&gt;Quercus ilex&lt;/em&gt; subsp&lt;em&gt;. ilex&lt;/em&gt; and &lt;em&gt;Q.&amp;nbsp;ilex&lt;/em&gt; subsp&lt;em&gt;. ballota&lt;/em&gt;) are the most representative tree species in the Iberian peninsula and the main tree species in oak-rangeland ecosystems (&lt;em&gt;dehesas&lt;/em&gt;). Oak decline in western, central and southern parts of Spain has been associated with root rot caused by &lt;em&gt;Phytophthora cinnamomi&lt;/em&gt; for decades. However, &lt;em&gt;Phytophthora&lt;/em&gt; species such as &lt;em&gt;P.&amp;nbsp;&lt;/em&gt;&amp;nbsp;&lt;em&gt;quercina&lt;/em&gt; and &lt;em&gt;P.&amp;nbsp;psychrophila&lt;/em&gt; have recently been found associated with &lt;em&gt;Quercus&lt;/em&gt; decline in eastern Spain where calcareous soils are predominant. Soil and root samples from two &lt;em&gt;Quercus&lt;/em&gt; forests presenting decline symptoms in two different geographical areas in eastern Spain (Carrascar de la Font Roja and Vallivana) were analysed by amplicon pyrosequencing. Metabarcoding analysis showed &lt;em&gt;Phytophthora&lt;/em&gt; species diversity, and revealed that an uncultured &lt;em&gt;Phytophthora&lt;/em&gt; taxon, named provisionally &lt;em&gt;Phytophthora&lt;/em&gt; taxon ballota, was the predominant species in both areas. In addition, a real-time PCR assay, based on the pyrosequencing results, was developed for the detection of this uncultured &lt;em&gt;Phytophthora&lt;/em&gt; taxon, and also for the detection of &lt;em&gt;P.&amp;nbsp;quercina&lt;/em&gt;. TaqMan assays were tested on soil and root samples, and on &lt;em&gt;Phytophthora&lt;/em&gt; pure cultures. The new assays showed high specificity and were consistent with metabarcoding results. A new real-time PCR protocol is proposed to evaluate the implication of different &lt;em&gt;Phytophthora&lt;/em&gt; spp. in oak decline in eastern Spain.&lt;/p&gt;</style></abstract></record></records></xml>