<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">König, S.</style></author><author><style face="normal" font="default" size="100%">Schwenkbier, L.</style></author><author><style face="normal" font="default" size="100%">Pollok, S.</style></author><author><style face="normal" font="default" size="100%">Riedel, M.</style></author><author><style face="normal" font="default" size="100%">Wagner, S.</style></author><author><style face="normal" font="default" size="100%">Popp, J.</style></author><author><style face="normal" font="default" size="100%">Weber, K.</style></author><author><style face="normal" font="default" size="100%">Werres, S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Potential of Ypt1 and ITS gene regions for the detection of Phytophthora species in a lab-on-a-chip DNA hybridization array</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Pathol</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-10-2015</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1111/ppa.2015.64.issue-5http://doi.wiley.com/10.1111/ppa.12357http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fppa.12357</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">64</style></volume><pages><style face="normal" font="default" size="100%">1176 - 1189</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;A novel DNA-chip hybridization assay that uses the &lt;em&gt;ras&lt;/em&gt;-related GTP-binding protein 1 gene (&lt;em&gt;Ypt1&lt;/em&gt;) was developed for the identification of several devastating &lt;em&gt;Phytophthora&lt;/em&gt; species. The hybridization was conducted in a portable microfluidic lab-on-a-chip device for fast and accurate detection of 40 &lt;em&gt;Phytophthora&lt;/em&gt;, two &lt;em&gt;Pythium&lt;/em&gt; and one &lt;em&gt;Phytopythium&lt;/em&gt; species. Moreover, the functionality of the &lt;em&gt;Ypt1&lt;/em&gt; region was examined in comparison to an array for the internal transcribed spacer (ITS) region by &lt;em&gt;in silico&lt;/em&gt; modelling. The difference in species-specific capture probe sequences was lower for the ITS than for the &lt;em&gt;Ypt1&lt;/em&gt; region. While ITS-probes of &lt;em&gt;Phytophthora ramorum&lt;/em&gt;,&lt;em&gt; Phytophthora fragariae&lt;/em&gt; and &lt;em&gt;Phytophthora lateralis&lt;/em&gt; cross-reacted with up to 11 non-target species, &lt;em&gt;Ypt1&lt;/em&gt;-probes were specific except for &lt;em&gt;P.&amp;nbsp;fragariae&lt;/em&gt;/&lt;em&gt;Phytophthora rubi&lt;/em&gt;. First analyses of artificially inoculated &lt;em&gt;Rhododendron&lt;/em&gt; leaves successfully demonstrated the usability of the respective capture probes for the &lt;em&gt;Ypt1&lt;/em&gt; and the &lt;em&gt;ras&lt;/em&gt;-related plant protein &lt;em&gt;Rab1a&lt;/em&gt; gene region. The on-chip hybridization enabled the detection of up to 1&amp;nbsp;pg&amp;nbsp;&lt;em&gt;μ&lt;/em&gt;L&lt;sup&gt;−1&lt;/sup&gt; target DNA depending on the species examined. Due to the complementarity of ITS and &lt;em&gt;Ypt1&lt;/em&gt; genetic features, the use of multiple loci is recommended to identify targets of different taxonomic rank.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue></record></records></xml>