<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">King, K. M.</style></author><author><style face="normal" font="default" size="100%">Harris, A. R.</style></author><author><style face="normal" font="default" size="100%">Webber, J. F.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In planta detection used to define the distribution of the European lineages of Phytophthora ramorum on larch (Larix ) in the UK</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Pathol</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2015</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-10-2015</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1111/ppa.2015.64.issue-5http://doi.wiley.com/10.1111/ppa.12345http://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fppa.12345</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">64</style></volume><pages><style face="normal" font="default" size="100%">1168 - 1175</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Two genetically distinct evolutionary lineages of the oomycete pathogen &lt;em&gt;Phytophthora ramorum&lt;/em&gt; are responsible for the major epidemic on larch (&lt;em&gt;Larix&lt;/em&gt; spp.) in the UK: EU1 (historically widespread) and the recently identified EU2 (reported only from Northern Ireland and a small area in southwest Scotland). Methods for lineage discrimination have required pure cultures of &lt;em&gt;P.&amp;nbsp;ramorum&lt;/em&gt; but, as the pathogen is challenging to isolate from infected larch tissue, only limited data have been available on the distribution of EU2. In this study a protocol was developed to determine the lineage of &lt;em&gt;P.&amp;nbsp;ramorum&lt;/em&gt; in infected larch tissue without the need for isolation. The protocol was applied to 134 UK samples collected during 2013–14. In addition, lineage testing was applied to over 300 &lt;em&gt;P.&amp;nbsp;ramorum&lt;/em&gt; isolates cultured from a wide range of hosts between 2002 and 2012. Combined data confirmed that EU2 is restricted to Northern Ireland and a small area of southwest Scotland where it is the dominant lineage. There was no evidence of EU2 spread into England and Wales where only EU1 was found. However, EU2 was more widely distributed in southern and eastern parts of Scotland than previously reported. Furthermore, EU1 and EU2 were detected &amp;lt;10&amp;nbsp;km apart in larch plantations. This study provides the first reports of natural EU2 infection on European larch (&lt;em&gt;Larix decidua&lt;/em&gt;), hybrid larch (&lt;em&gt;Larix&amp;nbsp;&lt;/em&gt;× &lt;em&gt;eurolepis&lt;/em&gt;), beech (&lt;em&gt;Fagus sylvatica&lt;/em&gt;), noble fir (&lt;em&gt;Abies procera&lt;/em&gt;) and western hemlock (&lt;em&gt;Tsuga heterophylla&lt;/em&gt;).&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue></record></records></xml>