<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Moralejo, E.</style></author><author><style face="normal" font="default" size="100%">Puig, M.</style></author><author><style face="normal" font="default" size="100%">Man in't Veld, W. A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">First report of Phytophthora tentaculata on Verbena sp. in Spain</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Pathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Pathology</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan-12-2004</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.blackwell-synergy.com/toc/ppa/53/6http://doi.wiley.com/10.1111/j.1365-3059.2004.01089.xhttp://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-3059.2004.01089.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">53</style></volume><pages><style face="normal" font="default" size="100%">806 - 806</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;div id=&quot;ss3&quot; class=&quot;section&quot;&gt;&lt;div class=&quot;para&quot;&gt;&lt;p&gt;The oomycete &lt;em&gt;Phytophthora tentaculata&lt;/em&gt; causes root and stalk rot of &lt;em&gt;Chrysanthemum&lt;/em&gt; spp., &lt;em&gt;Delphinium ajacis&lt;/em&gt; and &lt;em&gt;Verbena&lt;/em&gt; spp. in nurseries in the Netherlands and Germany (&lt;a class=&quot;referenceLink&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com.ezproxy.proxy.library.oregonstate.edu/doi/10.1111/j.1365-3059.2004.01089.x/full#b1&quot; rel=&quot;references:#b1&quot;&gt;Kröber &amp;amp; Marwitz, 1993&lt;/a&gt;). In June 2001, &lt;em&gt;P. tentaculata&lt;/em&gt; was isolated from a young potted &lt;em&gt;Verbena&lt;/em&gt; hybrid, showing a collar and stalk rot, in a nursery in Majorca (Balearic Islands, Spain). It was initially recovered by plating ∼10&amp;nbsp;mm pieces of necrotic tissue from the leading lesion on to a phytophthora selective medium, P&lt;sub&gt;5&lt;/sub&gt;ARP (&lt;a class=&quot;referenceLink&quot; title=&quot;Link to bibliographic citation&quot; href=&quot;http://onlinelibrary.wiley.com.ezproxy.proxy.library.oregonstate.edu/doi/10.1111/j.1365-3059.2004.01089.x/full#b2&quot; rel=&quot;references:#b2&quot;&gt;Erwin &amp;amp; Ribeiro, 1996&lt;/a&gt;). A pure culture (isolate CBS 115458) was obtained by transferring aseptically a hyphal tip onto corn meal agar (CMA) and was first identified from morphological characters.&lt;/p&gt;&lt;/div&gt;&lt;div class=&quot;para&quot;&gt;&lt;p&gt;The colony surface texture was uniform and formed sparse, loosely branched mycelium on carrot piece agar (CPA: 50&amp;nbsp;g carrot pieces and 20&amp;nbsp;g agar per 1000&amp;nbsp;mL distilled water) and CMA. The radial growth rate was 2–3&amp;nbsp;mm&amp;nbsp;day&lt;sup&gt;−1&lt;/sup&gt; at 20°C on CMA. Sporangia did not appear on either agar media but formed readily in soil extract (50&amp;nbsp;g soil from a holm oak forest suspended in 1&amp;nbsp;L ionized water for 24 h at 20°C and then filtered and autoclaved). The sporangia were ovoid to globose, 27–52 (36·9)&amp;nbsp;×&amp;nbsp;17–31 (24·6) &lt;em&gt;µ&lt;/em&gt;m, length:breadth ratio 1·4, papillate with a narrow exit pore, and some were caducous with a short pedicel (&amp;lt;&amp;nbsp;5&amp;nbsp;&lt;em&gt;µ&lt;/em&gt;m). Hyphal swellings were present in water. Chlamydospores were only seen on CMA after 2 weeks. Oogonia, readily produced on CPA in pure culture, were globose, mostly terminal or a few lateral, and ranged from 17 to 41 (34·0) &lt;em&gt;µ&lt;/em&gt;m in diameter. Single paragynous, monoclinous or diclinous, usually long-stalked antheridia were club-shaped or spherical, 9–16 (12·7) &lt;em&gt;µ&lt;/em&gt;m in diameter and many had appendages. Occasionally two paragynous antheridia per oogonium, as well as some amphigynous antheridia, were observed. Oospores were aplerotic 16–33 (28·4)&amp;nbsp;&lt;em&gt;µ&lt;/em&gt;m in diameter and thin-walled.&lt;/p&gt;&lt;/div&gt;&lt;div class=&quot;para&quot;&gt;&lt;p&gt;To further confirm its identity, isozyme analysis based on malate dehydrogenase (EC 1.1.1.37) and malic enzymes (EC 1.1.1.40) was performed. Isozyme profiles fitted exactly those of three &lt;em&gt;P. tentaculata&lt;/em&gt; strains: two strains ex-&lt;em&gt;Chrysanthemum&lt;/em&gt; (including CBS 552.96 paratype) and one strain ex-&lt;em&gt;Verbena&lt;/em&gt;; and differing from those of all other papillate species (&lt;a class=&quot;referenceLink&quot; title=&quot;Link to bibliographic citations&quot; href=&quot;http://onlinelibrary.wiley.com.ezproxy.proxy.library.oregonstate.edu/doi/10.1111/j.1365-3059.2004.01089.x/full#b3&quot; rel=&quot;references:#b3 #b4&quot;&gt;Oudemans &amp;amp; Coffey, 1991a,b&lt;/a&gt;). Pathogenicity was assessed by flooding three potted &lt;em&gt;Verbena&lt;/em&gt; plants with a 10&lt;sup&gt;4&lt;/sup&gt; mL&lt;sup&gt;−1&lt;/sup&gt; zoospore suspension for 48 h at 20–22°C. As controls, two potted &lt;em&gt;Verbena&lt;/em&gt; plants were flooded with ionized water. Controls remained healthy 15&amp;nbsp;days after inoculation. All three inoculated &lt;em&gt;Verbena&lt;/em&gt; plants exhibited collar rot after 15&amp;nbsp;days, from which the pathogen was reisolated using PARP medium, thus confirming Koch's postulates. This is the first report of &lt;em&gt;P. tentaculata&lt;/em&gt; in Spain.&lt;/p&gt;&lt;/div&gt;&lt;/div&gt;</style></abstract><issue><style face="normal" font="default" size="100%">6</style></issue></record></records></xml>