<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Engelbrecht, J.</style></author><author><style face="normal" font="default" size="100%">Duong, T. A.</style></author><author><style face="normal" font="default" size="100%">van den Berg, N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a Nested Quantitative Real-Time PCR for Detecting Phytophthora cinnamomi in Persea americana Rootstocks</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Disease</style></secondary-title><short-title><style face="normal" font="default" size="100%">Plant Disease</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">08/2013</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1094/PDIS-11-12-1007-RE </style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">97</style></volume><pages><style face="normal" font="default" size="100%">1012 - 1017</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Phytophthora cinnamomi&lt;/em&gt; causes Phytophthora root rot (PRR) in avocado (&lt;em&gt;Persea americana&lt;/em&gt;), an important disease that causes severe economic losses to the avocado industry globally. To date, no PRR-resistant avocado rootstock variety has been discovered, although certain rootstock varieties have been shown to be more tolerant than others. In this study, we developed an accurate, low cost assay for in planta quantification of &lt;em&gt;P. cinnamomi&lt;/em&gt; to evaluate disease tolerance. A nested real-time polymerase chain reaction assay was developed to sensitively detect pathogen DNA in plant tissues. Root samples from a highly tolerant (Dusa) and less tolerant (R0.12) rootstock were collected at 0, 3, 7, 14, and 21 days after inoculation with &lt;em&gt;P. cinnamomi&lt;/em&gt; and used for pathogen quantification. Nested primers developed in this study were specific and sensitive and could detect &lt;em&gt;P. cinnamomi&lt;/em&gt; in root tissues. The amount of &lt;em&gt;P. cinnamomi&lt;/em&gt; quantified in roots was significantly higher in the less-tolerant R0.12 plants when compared with the highly tolerant Dusa plants at all time points. This study has confirmed the known status of disease tolerance of Dusa and R0.12 avocado rootstocks in a quantitative manner and provides a reliable molecular tool to assist with industry breeding programs for the selection of PRR-resistant avocado rootstock varieties.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue></record></records></xml>