<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chen, Wen</style></author><author><style face="normal" font="default" size="100%">Djama, Zeinab Robleh</style></author><author><style face="normal" font="default" size="100%">Michael D. Coffey</style></author><author><style face="normal" font="default" size="100%">Martin, Frank N.</style></author><author><style face="normal" font="default" size="100%">Bilodeau, Guillaume J.</style></author><author><style face="normal" font="default" size="100%">Radmer, Lorien</style></author><author><style face="normal" font="default" size="100%">Denton, Geoff</style></author><author><style face="normal" font="default" size="100%">Lévesque, C. André</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Membrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species</style></title><secondary-title><style face="normal" font="default" size="100%">Phytopathology</style></secondary-title><short-title><style face="normal" font="default" size="100%">Phytopathology</style></short-title></titles><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">01/2013</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">103</style></volume><pages><style face="normal" font="default" size="100%">43 - 54</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Most &lt;em&gt;Phytophthora&lt;/em&gt; spp. are destructive plant pathogens; therefore, effective monitoring and accurate early detection are important means of preventing potential epidemics and outbreaks of diseases. In the current study, a membrane-based oligonucleotide array was developed that can detect &lt;em&gt;Phytophthora&lt;/em&gt; spp. reliably using three DNA regions; namely, the internal transcribed spacer (ITS), the 5′ end of cytochrome &lt;em&gt;c&lt;/em&gt; oxidase 1 gene (&lt;em&gt;cox&lt;/em&gt;1), and the intergenic region between cytochrome &lt;em&gt;c&lt;/em&gt; oxidase 2 gene (&lt;em&gt;cox&lt;/em&gt;2) and &lt;em&gt;cox&lt;/em&gt;1 (&lt;em&gt;cox&lt;/em&gt;2-1 &lt;em&gt;spacer&lt;/em&gt;). Each sequence data set contained ≈250 sequences representing 98 described and 15 undescribed species of &lt;em&gt;Phytophthora&lt;/em&gt;. The array was validated with 143 pure cultures and 35 field samples. Together, nonrejected oligonucleotides from all three markers have the ability to reliably detect 82 described and 8 undescribed &lt;em&gt;Phytophthora&lt;/em&gt; spp., including several quarantine or regulated pathogens such as &lt;em&gt;Phytophthora ramorum&lt;/em&gt;. Our results showed that a DNA array containing signature oligonucleotides designed from multiple genomic regions provided robustness and redundancy for the detection and differentiation of closely related taxon groups. This array has the potential to be used as a routine diagnostic tool for &lt;em&gt;Phytophthora&lt;/em&gt; spp. from complex environmental samples without the need for extensive growth of cultures.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue></record></records></xml>