<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hughes, KelvinJ.D.</style></author><author><style face="normal" font="default" size="100%">Tomlinson, JennyA.</style></author><author><style face="normal" font="default" size="100%">Giltrap, PatriciaM.</style></author><author><style face="normal" font="default" size="100%">Barton, Victoria</style></author><author><style face="normal" font="default" size="100%">Hobden, Ellie</style></author><author><style face="normal" font="default" size="100%">Boonham, Neil</style></author><author><style face="normal" font="default" size="100%">Lane, CharlesR.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a real-time PCR assay for detection of Phytophthora kernoviae and comparison of this method with a conventional culturing technique</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Plant Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Diagnostic sensitivity</style></keyword><keyword><style  face="normal" font="default" size="100%">Diagnostic specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">plant health</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhododendron</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1007/s10658-011-9843-x</style></url></web-urls></urls><publisher><style face="normal" font="default" size="100%">Springer Netherlands</style></publisher><volume><style face="normal" font="default" size="100%">131</style></volume><pages><style face="normal" font="default" size="100%">695-703</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em class=&quot;a-plus-plus&quot;&gt;Phytophthora kernoviae&lt;/em&gt; is a recently described pathogen causing leaf blight, aerial dieback and bleeding cankers on trees and shrubs in parts of Great Britain and Ireland and recently reported in New Zealand. This paper describes the development of a TaqMan real-time PCR assay based on internal transcribed spacer (ITS) sequence to aid diagnosis of this pathogen in culture and in plant material. The assay showed no cross reaction with 29 other &lt;em class=&quot;a-plus-plus&quot;&gt;Phytophthora&lt;/em&gt; species, including the closely related species &lt;em class=&quot;a-plus-plus&quot;&gt;P. boehmeriae&lt;/em&gt;, and detected at least 1.2&amp;nbsp;pg of &lt;em class=&quot;a-plus-plus&quot;&gt;P. kernoviae&lt;/em&gt; DNA per reaction. A rapid and simple method can be used to extract DNA prior to testing by real-time PCR, and a plant internal control assay can be used to aid interpretation of negative results. A comparison of real-time PCR and plating for 526 plant samples collected in the UK indicated that this assay is suitable for use in routine screening for &lt;em class=&quot;a-plus-plus&quot;&gt;P. kernoviae&lt;/em&gt;.&lt;/p&gt;</style></abstract></record></records></xml>