<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Elliott, M.</style></author><author><style face="normal" font="default" size="100%">Sumampong, G.</style></author><author><style face="normal" font="default" size="100%">Varga, A.</style></author><author><style face="normal" font="default" size="100%">Shamoun, S. F.</style></author><author><style face="normal" font="default" size="100%">James, D.</style></author><author><style face="normal" font="default" size="100%">Masri, S.</style></author><author><style face="normal" font="default" size="100%">Brière, S. C.</style></author><author><style face="normal" font="default" size="100%">N. J. Grünwald</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">PCR-RFLP markers identify three lineages of the North American and European populations of Phytophthora ramorum</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.1439-0329.2008.00586.x</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">4</style></number><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">266–278</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Phytophthora ramorum&lt;/em&gt;, the cause of sudden oak death and ramorum blight, has three major clonal lineages and two mating types. Molecular tests currently available for detecting &lt;em&gt;P. ramorum&lt;/em&gt; do not distinguish between clonal lineages and mating type is determined by cultural methods on a limited number of samples. In some molecular diagnostic tests, cross-reaction with other closely related species such as &lt;em&gt;P. hibernalis&lt;/em&gt;, &lt;em&gt;P. foliorum&lt;/em&gt; or &lt;em&gt;P. lateralis&lt;/em&gt; can occur. Regions in the mitochondrial gene &lt;em&gt;Cox1&lt;/em&gt; are different among &lt;em&gt;P. ramorum&lt;/em&gt; lineages and mitochondrial genotyping of the North American and European populations seems to be sufficient to differentiate between mating types, because the EU1 lineage is mostly A1 and both NA1 and NA2 lineages are A2. In our study, we were able to identify &lt;em&gt;P. ramorum&lt;/em&gt; isolates according to lineage using polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) of the &lt;em&gt;Cox1&lt;/em&gt; gene, first by using &lt;em&gt;Apo&lt;/em&gt;I to separate &lt;em&gt;P. ramorum&lt;/em&gt; from other species and EU1 from North American populations, and then &lt;em&gt;Ava&lt;/em&gt;I to distinguish between NA1 and NA2 genotypes. However, &lt;em&gt;P. foliorum&lt;/em&gt; had the same restriction profile as &lt;em&gt;P. ramorum&lt;/em&gt; NA1 isolates.&lt;/p&gt;</style></abstract></record></records></xml>