<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Durán, Alvaro</style></author><author><style face="normal" font="default" size="100%">Bernard Slippers</style></author><author><style face="normal" font="default" size="100%">Marieka Gryzenhout</style></author><author><style face="normal" font="default" size="100%">Rodrigo Ahumada</style></author><author><style face="normal" font="default" size="100%">Drenth, Andre</style></author><author><style face="normal" font="default" size="100%">Brenda D. Wingfield</style></author><author><style face="normal" font="default" size="100%">Michael J. Wingfield</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">DNA-based method for rapid identification of the pine pathogen, Phytophthora pinifolia</style></title><secondary-title><style face="normal" font="default" size="100%">FEMS Microbiology Letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Monterey pine</style></keyword><keyword><style  face="normal" font="default" size="100%">oomycetes</style></keyword><keyword><style  face="normal" font="default" size="100%">Pinus radiata</style></keyword><keyword><style  face="normal" font="default" size="100%">tree defoliation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2009.01700.x/abstract</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">298</style></volume><pages><style face="normal" font="default" size="100%">99-104</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;em&gt;Phytophthora pinifolia&lt;/em&gt; causes a needle and shoot disease in &lt;em&gt;Pinus radiata&lt;/em&gt;, referred to as ‘Daño Foliar del Pino’. This newly discovered disease requires intensive research efforts that necessitate the processing of large numbers of samples for which accurate identification, often by people not experienced in &lt;em&gt;Phytophthora&lt;/em&gt; taxonomy, is required. The aim of this study was, therefore, to develop species-specific primers for &lt;em&gt;P. pinifolia&lt;/em&gt; that amplify the internal transcribed spacer region of the ribosomal operon and the nuclear Ypt1 gene, respectively. The primers were tested over several &lt;em&gt;Phytophthora&lt;/em&gt; spp., as well as fungi isolated from &lt;em&gt;P. radiata&lt;/em&gt;. In all cases, only &lt;em&gt;P. pinifolia&lt;/em&gt; was amplified. In addition to the species-specific primers, a PCR-restriction fragment length polymorphism protocol using available &lt;em&gt;Phytophthora&lt;/em&gt; genus-specific primers was also used to generate a species-specific profile for &lt;em&gt;P. pinifolia&lt;/em&gt;. This provided a characteristic profile that allows the identification of &lt;em&gt;P. pinifolia&lt;/em&gt;, and it could also discriminate between 27 different species of &lt;em&gt;Phytophthora&lt;/em&gt;. Both techniques reported in this study make it possible to identify large numbers of &lt;em&gt;P. pinifolia&lt;/em&gt; cultures accurately and efficiently, which will be important for both quarantine work and biological research on this important new pathogen.&lt;/p&gt;</style></abstract></record></records></xml>