<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bakonyi, J.</style></author><author><style face="normal" font="default" size="100%">Nagy, Z. Á.</style></author><author><style face="normal" font="default" size="100%">Érsek, T.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">PCR-based DNA Markers for identifying hybrids within Phytophthora alni</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Phytopathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Alnus</style></keyword><keyword><style  face="normal" font="default" size="100%">oomycete</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytophthora alni</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction markers</style></keyword><keyword><style  face="normal" font="default" size="100%">species hybrids</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.1439-0434.2006.01079.x</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><publisher><style face="normal" font="default" size="100%">Blackwell Verlag GmbH</style></publisher><volume><style face="normal" font="default" size="100%">154</style></volume><pages><style face="normal" font="default" size="100%">168–177</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Two pairs of oligonucleotide primers were designed for the polymerase chain reaction (PCR)-based detection and differential identification of naturally occurring interspecific hybrid types (subspecies) of &lt;em&gt;Phytophthora alni&lt;/em&gt;, all of which cause collar rot of alder trees. Primer pairs were derived from randomly amplified polymorphic DNA (RAPD) fragments that were unique to various subspecies of this alder pathogen. The primer pair set, SAP1/SAP2 (SAP), was derived from a 0.93-kb RAPD fragment amplified from &lt;em&gt;P. alni&lt;/em&gt; ssp. &lt;em&gt;alni&lt;/em&gt;. The primer pair set, SWAP1/SWAP2 (SWAP), was derived from a 1.13-kb fragment amplified from &lt;em&gt;P. alni&lt;/em&gt; ssp. &lt;em&gt;uniformis&lt;/em&gt;. Patterns of SAP and SWAP amplification enabled distinction among the three subspecies. No PCR products were amplified from isolates of 31 other &lt;em&gt;Phytophthora&lt;/em&gt; spp. examined, including &lt;em&gt;P. cambivora&lt;/em&gt; and &lt;em&gt;P. fragariae&lt;/em&gt;, the suspected progenitors of &lt;em&gt;P. alni&lt;/em&gt;. The SAP and SWAP primer sets were able to detect a minimum of 10&amp;nbsp;pg of DNA from pure cultures or DNA extracted from as few as 10 zoospores. Pathogen DNA could also be amplified directly from bark lesions of artificially inoculated and naturally infected common alders and from lesions developed on common cherry-laurel leaves used in baiting the pathogen from infested soil. Direct detection of pathogen DNA from alder tissue using SAP and SWAP primer sets should prove useful in developing measures for effective quarantine and management of &lt;em&gt;P. alni&lt;/em&gt;.&lt;/p&gt;</style></abstract></record></records></xml>