TY - JOUR T1 - Enhanced recovery of Phytophthora ramorum from soil following 30 Days of storage at 4°C JF - Journal of Phytopathology Y1 - 2011 A1 - Tooley, Paul W. A1 - Carras, Marie M. KW - chlamydospore KW - ramorum blight AB -

Chlamydospores of Phytophthora ramorum were used to infest field soil at densities ranging from 0.2 to 42 chlamydospores/cm3 soil. Recovery was determined by baiting with rhododendron leaf discs and dilution plating at time 0 and after 30 days of storage at 4°C, as recommended by USDA-APHIS. Baiting was slightly more sensitive than dilution plating in recovering P. ramorum immediately following infestation of soil and allowed detection from samples infested with as little as 0.2 chlamydospores/cm3 compared with 1 chlamydospore/cm3 for dilution plating. After 30 days of infested soil storage at 4°C, P. ramorum was detected at significantly (P = 0.05) higher levels than at time 0 with both recovery methods. The results indicate that storage of P. ramorum-infested soil at 4°C may allow for pathogen activity, such as sporangia production, which may enhance recovery from soil.

PB - Blackwell Publishing Ltd UR - http://dx.doi.org/10.1111/j.1439-0434.2011.01810.x ER -