01821nas a2200145   4500008004100000022001400041245007200055210006900127300001400196490000800210520131400218653002001532100002301552856010001575       2010                        eng d  a1878-614600aPhytophthora kernoviae oospore maturity, germination, and infection0 aPhytophthora kernoviae oospore maturity germination and infectio  a661 - 6680 v1143 a<p>Limited information is known on the basic biology of the recently described <em>Phytophthora kernoviae</em> that produces homothallic oospores. In this study, different <em>P. kernoviae</em> isolates were used to investigate oospore maturity, germination, and infection. All isolates produced oospores in V8 broth at 20†$ınfty$C in the dark by 6†d. Oospores also formed at 10 and 15†$ınfty$C, but did not form at 25 and 28†$ınfty$C. Continuous light inhibited oospore production of some isolates but had no negative effect on others. Maturation time of the oospores, as noted by germination and staining with tetrazolium bromide, was not much different among the isolates between 2 and 14 weeks. Oospore germination was optimal at 18 and 20†$ınfty$C, and did not occur at 5, 25, and 30†$ınfty$C. Oospore germination under continuous light was higher than in the dark, but individual isolates showed variable results. <em>Rhododendron</em> leaf disks inoculated with oospores and maintained in the dark at 20†$ınfty$C were necrotic after 1 week, while those kept under continuous light did not develop necrosis. The percentage of leaf disks infected with <em>P. kernoviae</em> was lower in the leaves exposed to continuous light (40†%) compared to those kept in the dark (100†%).</p>10aViability stain1 aWidmer, Timothy, L  uhttp://www.sciencedirect.com/science/article/B9879-508K88P-1/2/e35155432b3146cdabc05226a599fa75