01902nas a2200253   4500008004100000022001400041245007500055210006900130300001600199490000700215520113900222653001401361100001901375700001801394700002101412700002001433700002301453700001901476700002401495700001901519700002001538700001901558856007101577       2011                        eng d  a0956-566300aDevelopment of a lab-on-a-chip device for diagnosis of plant pathogens0 aDevelopment of a labonachip device for diagnosis of plant pathog  a4070 - 40750 v263 a<p>A lab-on-a-chip system for rapid nucleic acid-based analysis was developed that can be applied for diagnosis of selected <em>Phytophthora</em> species as a first example for use in plant pathology. All necessary polymerase chain reaction process (PCR) and hybridization steps can be performed consecutively within a single chip consisting of two components, an inflexible and a flexible one, with integrated microchannels and microchambers. Data from the microarray is collected from a simple electrical measurement that is based on elementary silver deposition by enzymatical catalyzation. Temperatures in the PCR and in the hybridization zone are managed by two independent Peltier elements. The chip will be integrated in a compact portable system with a pump and power supply for use on site. The specificity of the lab-on-a-chip system could be demonstrated for the tested five <em>Phytophthora</em> species. The two <em>Pythium</em> species gave signals below the threshold. The results of the electrical detection of the microarray correspond to the values obtained with the control method (optical grey scale analysis).</p>10adetection1 aJulich, Sandra1 aRiedel, Marko1 aKielpinski, Mark1 aUrban, Matthias1 aKretschmer, Robert1 aWagner, Stefan1 aFritzsche, Wolfgang1 aHenkel, Thomas1 aMöller, Robert1 aWerres, Sabine  uhttp://www.sciencedirect.com/science/article/pii/S0956566311001916