02726nas a2200169   4500008004100000022001400041245009100055210006900146260001600215300001100231490000800242520217900250100002502429700002102454700002502475856005602500       2016                        eng d  a0027-551400aPhytophthora species recovered from the Connecticut River Valley in Massachusetts, USA0 aPhytophthora species recovered from the Connecticut River Valley  cAug-01-2017  a6 - 190 v1083 a<p>Little is currently known about the assemblage of <em>Phytophthora</em> species in northeastern North America, representing a gap in our understanding of species incidence. Therefore, <em>Phytophthora</em> species were surveyed at 20 sites in Massachusetts, with 16 occurring in the Connecticut River Valley. Many of the sampled waterways were adjacent to active agricultural lands, yet were buffered by mature floodplain forests composed of <em>Acer, Platanus, Populus</em> and <em>Ulmus</em>. Isolates were recovered with three types of baits (rhododendron leaves, pear, green pepper) in 2013 and water filtration in 2014. Overall, 457 isolates of <em>Phytophthora</em> were recovered and based on morphological characters and rDNA internal transcribed spacer (ITS), β-tubulin (β-<em>tub</em>) and cytochrome oxidase <em>c</em> subunit I (<em>cox</em>1) sequences, 18 taxa were identified, including three new species: <em>P.</em> taxon intercalaris, <em>P.</em> taxon caryae and <em>P.</em> taxon pocumtuck. In addition, 49 isolates representing five species of <em>Phytopythium</em> also were identified. Water filtration captured a greater number of taxa (18) compared to leaf and fruit baits (12). Of the three bait types rhododendron leaves yielded the greatest number of isolates and taxa, followed by pear and green pepper, respectively. Despite the proximity to agricultural lands, none of the <em>Phytophthora</em> species baited are considered serious pathogens of vegetable crops in the region. However, many of the recovered species are known woody plant pathogens, including four species in the <em>P. citricola</em> s.l. complex that were identified: <em>P. plurivora, P. citricola</em> III, <em>P. pini</em> and a putative novel species, referred to here as <em>P.</em> taxon caryae. An additional novel species, <em>P.</em> taxon pocumtuck, is a close relative of <em>P. borealis</em> based on <em>cox</em>1 sequences. The results illustrate a high level of <em>Phytophthora</em> species richness in the Connecticut River Valley and that major rivers can serve as a source of inoculum for pathogenic <em>Phytophthora</em> species in the northeast.</p>1 aBrazee, Nicholas, J.1 aWick, Robert, L.1 aHulvey, Jonathan, P.  uhttps://www.tandfonline.com/doi/full/10.3852/15-038