01617nas a2200193 4500008004100000245011500041210006900156260001200225300001400237490000800251520093800259100001901197700001901216700002101235700002501256700002701281700001901308856009601327 2014 eng d00aThe Microscopic Examination of Phytophthora cinnamomi in Plant Tissues Using Fluorescent In Situ Hybridization0 aMicroscopic Examination of Phytophthora cinnamomi in Plant Tissu c12/2014 a747 - 7570 v1623 a

The microscopic examination of Phytophthora cinnamomi in plant tissues is often difficult as structures such as hyphae, chlamydospores and oospores are frequently indistinguishable from those of other fungi and oomycetes, with histological stains not enabling species differentiation. This lack of staining specificity makes the localization of P. cinnamomi hyphae and reproductive structures within plant tissues difficult, especially in woody tissues. This study demonstrates that with the use of a species-specific fluorescently labelled DNA probe, P. cinnamomi can be specifically detected and visualized directly using fluorescent in situ hybridization (FISH) without damage to plant or pathogen cell integrity or the need for subculturing. This approach provides a new application for FISH with potential use in the detailed study of plant–pathogen interactions in plants.

1 aLi, Andrew, Y.1 aCrone, Michael1 aAdams, Peter, J.1 aFenwick, Stanley, G.1 aHardy, Giles, E. S. J.1 aWilliams, Nari uhttp://doi.wiley.com/10.1111/jph.2014.162.issue-11-12http://doi.wiley.com/10.1111/jph.12257