01691nas a2200205   4500008004100000245011700041210006900158300001400227490000700241520104400248100001601292700001401308700001401322700001501336700001401351700001801365700001601383700001401399856007201413       2012                        eng d00aMicrosatellite markers for population studies of Phytophthora megakarya (Pythiaceae), a cacao pathogen in Africa0 aMicrosatellite markers for population studies of Phytophthora me  ae353-e3560 v993 a<p>‚Ä¢ Premise of the study: Phytophthora megakarya is the agent of black pod disease of cacao and is the main pathogen of this crop in Africa. Population genetic studies are required to investigate how this pathogen emerged. To this end, we developed 12 novel polymorphic microsatellite markers for P. megakarya.‚Ä¢ Methods and Results: Microsatellite sequences were obtained by pyrosequencing of multiplex-enriched libraries. Candidate loci with di- or trinucleotide motifs were selected, and primer pairs were tested with nine P. megakarya isolates. The 12 most polymorphic and unambiguous loci were selected to develop three multiplex PCR pools. The total number of alleles varied from two to nine, depending on loci, and higher than expected heterozygosity was observed.‚Ä¢ Conclusions: These markers were used for population genetic studies of P. megakarya in Cameroon and for comparison with reference strains from West Africa. This is the first time that microsatellite markers have been developed for P. megakarya.</p>1 aMfegue, C V1 aHerail, C1 aAdreit, H1 aMbenoun, M1 aTechou, Z1 aTen Hoopen, M1 aTharreau, D1 aDucamp, M  uhttp://www.amjbot.org/content/early/2012/08/29/ajb.1200053.abstract