@article {JPH:JPH1810, title = {Enhanced recovery of Phytophthora ramorum from soil following 30 Days of storage at 4{\textdegree}C}, journal = {Journal of Phytopathology}, year = {2011}, pages = {no{\textendash}no}, publisher = {Blackwell Publishing Ltd}, abstract = {

Chlamydospores of Phytophthora ramorum were used to infest field soil at densities ranging from 0.2 to 42 chlamydospores/cm3 soil. Recovery was determined by baiting with rhododendron leaf discs and dilution plating at time 0 and after 30\ days of storage at 4{\textdegree}C, as recommended by USDA-APHIS. Baiting was slightly more sensitive than dilution plating in recovering P.\ ramorum immediately following infestation of soil and allowed detection from samples infested with as little as 0.2\ chlamydospores/cm3 compared with 1 chlamydospore/cm3 for dilution plating. After 30\ days of infested soil storage at 4{\textdegree}C, P.\ ramorum was detected at significantly (P\ =\ 0.05) higher levels than at time 0 with both recovery methods. The results indicate that storage of P.\ ramorum-infested soil at 4{\textdegree}C may allow for pathogen activity, such as sporangia production, which may enhance recovery from soil.

}, keywords = {chlamydospore, ramorum blight}, issn = {1439-0434}, doi = {10.1111/j.1439-0434.2011.01810.x}, url = {http://dx.doi.org/10.1111/j.1439-0434.2011.01810.x}, author = {Tooley, Paul W. and Carras, Marie M.} }